Click image to see more details
Product Info Summary
| SKU: | FC20056-PE |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human HLA-ABC Monoclonal Antibody PE Conjugated, Flow Validated
SKU/Catalog Number
FC20056-PE
Size
100 tests, 200 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human HLA-ABC Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC20056-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human HLA-ABC Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC20056-PE)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: W6/32
Isotype
IgG2a,k
Immunogen
Human Leukocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC20056-PE is reactive to in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Applications
FC20056-PE is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
12392 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated PBMCs stained with PE conjugated anti-human HLA-ABC (clone W6/32) (red histogram). Lymphocytes gated PBMCs stained with PE conjugated mouse IgG2a isotype control (Blue histogram).
Specific Publications For Anti-Human HLA-ABC Monoclonal Antibody PE Conjugated, Flow Validated (FC20056-PE)
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No publications found for FC20056-PE
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Human HLA-ABC Monoclonal Antibody PE Conjugated, Flow Validated
Question
Is a blocking peptide available for product anti-Human HLA-ABC Monoclonal antibody PE Conjugated, Flow Validated (FC20056-PE)?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
We do provide the blocking peptide for product anti-Human HLA-ABC Monoclonal antibody PE Conjugated, Flow Validated (FC20056-PE). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-08
Question
I see that the anti-Human HLA-ABC Monoclonal antibody PE Conjugated, Flow Validated FC20056-PE works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-03
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-03
Question
Would FC20056-PE anti-Human HLA-ABC Monoclonal antibody PE Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Johnson
Verified customer
Asked: 2017-09-28
Answer
You can see on the product datasheet, FC20056-PE anti-Human HLA-ABC Monoclonal antibody PE Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-28
